Color developmental conditions for LDH isoenzyme assay by agarose gel electrophoresis.
نویسندگان
چکیده
منابع مشابه
Agarose gel electrophoresis.
MATERIALS 6x Gel-loading buffer Agarose solutions (please see Step 3) DNA samples DNA size standards Samples of DNAs of known size are typically generated by restriction enzyme digestion of a plasmid or bacteriophage DNA of known sequence. Alternatively, they are produced by ligating a monomer DNA fragment of known size into a ladder of polymeric forms. DNA staining solution For a discussion of...
متن کاملAgarose gel electrophoresis.
Agarose gel electrophoresis is used to separate DNA or RNA molecules based upon their size.
متن کاملAlkaline agarose gel electrophoresis.
INTRODUCTION Alkaline agarose gels are run at a pH that is sufficiently high to denature double-stranded DNA. The denatured DNA is maintained in a single-stranded state and migrates through the alkaline gel as a function of its size. Alkaline agarose gels are used chiefly to measure the size of first and second strands of cDNA (Construction of cDNA Libraries Stage 1: Synthesis of First-strand c...
متن کاملResolving Transferrin Isoforms via Agarose Gel Electrophoresis.
OBJECTIVE Cerebrospinal fluid (CSF) leaks may present as rhinorrhea or otorrhea and can lead to life-threatening complications if not detected. The usefulness of the morpholinopropanesulfonic acid (MOPS)-histidine buffer in detecting β₂-transferrin, which is only found in the cerebrospinal fluid, was compared with the standard barbital buffer. METHODS We evaluated 20 aural or nasal patient sp...
متن کاملPrinciples of Nucleic Acid Separation by Agarose Gel Electrophoresis
Agarose gel electrophoresis is a routinely used method for separating proteins, DNA or RNA. (Kryndushkin et al., 2003). Nucleic acid molecules are size separated by the aid of an electric field where negatively charged molecules migrate toward anode (positive) pole. The migration flow is determined solely by the molecular weight where small weight molecules migrate faster than larger ones (Samb...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 1985
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.29.403